G3 Protocol
|
Age |
Test |
Primary parameters |
|---|---|---|
| 6wk | BCS* | Fibrinogen, ProC, FVIII |
| 7wk | Advia* | CBC |
| 8wk | Chem Analyzer* | FAST 4 hrs; Basal Plasma Lipids (Total Cholesterol, HDL, TG, Glucose) |
| CCMS (some) | ~One-third of all G3s; 3-day protocol; rest/activity, RER, food & water consumption | |
| 9wk | Begin Atherogenic Diet | |
| DLCO | Done same day as but prior to Buxco protocol | |
| BUXCO | Baseline, CO2, Methacholine | |
| 10wk | Blood Pressure | SBP, Pulse: train 3 days; measure 2 days |
| 11wk | BCS* | ATIII, FVIII |
| 12wk | PIXImus | Anesthetize (Avertin); Weight, BMD, BMC, % fat |
| 12.5 wk | Cardiac Ultrasound | Anesthetize (Pentobarbital/ETOH mix), M-Mode, 2D, Doppler |
| 13wk | Chem Analyzer* | FAST 4 hrs; Response to diet (Total Cholesterol, HDL, TG, Glucose) |
| 14wk | Obesity (ELISA)* |
NON-FASTED: ELISA; Plasma Insulin on glucose outliers @ 13 weeks; Plasma Leptin on % fat outliers identified @ 12wk PIXI |
| EKG | Conscious EKG | |
| 15wk | END | Remove from Atherogenic Diet and resume chow |
Note: Weights are taken at 8, 12 and 14 weeks. Weights are also taken for coagulopathy outliers at 18 weeks.
* Blood is collected via retro-orbital sinus in the non-fasted state, unless otherwise indicated.
Program for Genomic Applications (PGA)
Supported by the National Heart, Lung, and Blood Institute (NHLBI) (Grant # HL66611)
