HLBS Protocols

We welcome your input about the phenotyping screens we are currently performing as well as any suggestions for screens you would like us to undertake in the future. Please send us your comments .
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BP View Software Available - Our BP View software is designed to import data from the Visitech BP-2000 mouse blood pressure equipment (Visitech, Cary, NC) for a complete study at one time. The mouse identifier includes a suffix that denotes if the blood pressure measurement is a Training day or a Data day. The software allows for the review of the mouse identifiers and correction of errors in the incoming data. Two methods of statistical polishing of the data are provided. The data is then displayed with all data points available to the reviewer and statistics for the Data days displayed in a printable format. The software comes with documentation and a sample data set for testing and validation. The program runs in Microsoft Access 2000 and the documentation is in Microsoft Word format. The program works on Windows 98, XP, NT, or 2000 and will work on an Apple computer with a Windows emulator. For copies of the program package, please contact Dr. Randy Von Smith.

HTML Document Print format Images (If Available)
Animal environment parameters Word format N/A
Quality Assurance Word format N/A
Mutagenesis/Genetic Screening Strategies Word format N/A
ENU Treatment Protocol Word format N/A
EMS Treatment Protocol of ES Cells Word format N/A
Phenotyping Strategy for G3 Mice (text) 2001-2003 Word format N/A
Phenotyping Strategy for G3 Mice (graphic) 2001-2003 Word format N/A
Current Phenotyping Strategy for G3 Mice (graphic) 2004-2008 Flash format G3 Streamline Workflow Graphic (.jpg)
Heritability Protocol Flash format N/A

Assessment of Pulmonary Function: Whole Body Plethysmograph

 Word format Buxco Image #1
Buxco Bench Protocol N/A Buxco Image #2
Dade-Behring Blood Coagulation System Word format BCS Image

Blood Plasma Chemistry Analysis

 Word format N/A
Chemistry Bench Protocol Word format N/A
Assessment of Blood Pressure

Word format

 BP Image #1
BP Image #2
ADVIA 120 Hematology System N/A N/A

ADVIA Bench Protocol

 N/A N/A
ALPCO Diagnostics 1-2-3 UltraSensitive Mouse Insulin ELISA Word format N/A
ELISA Leptin Bench Protocol Word format N/A
Crystal Chem Leptin Protocol Word format N/A

R&D Quantikine Leptin Protocol

 Word format N/A

Bone Densitometry & Body Composition (PIXImus)

 Word format PIXI Image
PIXI Bench Protocol Word format N/A

Echocardiology/Cardiac Ultrasound

 Word format Echo Image
Isoflurane Protocol N/A N/A
Ultrasound Bench Protocol N/A N/A
Comprehensive Cage Monitoring System (CCMS) Word format CCMS Image #1
CCMS Image #2
Sleep Word format N/A
ECG Protocol Word format N/A
Atherogenic Diet Word format N/A

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Program for Genomic Applications (PGA)

Supported by the National Heart, Lung, and Blood Institute (NHLBI) (Grant # HL66611)