Animal environment parameters
Feed: All mices maintained on a 6% fat diet (Purina 5K52).
Purina 5K52
5K52: Cylinder shaped pellet - 3/8" diameter by 3/4" length.
PURINA QUALIFIED ANALYSIS
Crude protein not less than 18.0%
Crude fat not less than 6.0%
Crude fiber not more than 5.0%
Ash not more than 8.0%
Added minerals not more than 3.0%
INGREDIENTS
Ground wheat, ground corn, wheat middlings, ground oats, fish meal, dehulled soybean meal, soybean oil, corn gluten meal, dehydrated alfalfa meal, dicalcium phosphate, brewers dried yeast, calcium carbonate, menadione dimethylpyrimidinol bisulfite, salt, choline chloride, magnesium oxide, thiamin mononitrate, pyridoxine hydrochloride, cholecalciferol, vitamin A acetate, calcium pantothenate, ferrous sulfate, biotin, manganous oxide, cyanocobalamin, riboflavin, nicotinic acid, dl-alpha tocopheryl acetate, folic acid, calcium iodate, cobalt carbonate, zinc oxide, ferrous carbonate, zinc sulfate.
Water: Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/ml) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA).
Housing: Thoren cages; Thoren Maxi Miser #3 cages with a PIV (pressurized individually ventilated) system.
Bedding: Steam autoclaved white pine shavings from Crobb Box Co. of Ellsworth, ME.
Photoperiod: 12/12 Light/Dark; beginning at 6AM.
Temperature: 18 - 24 °C
Relative humidity: ~50%.
For more information in PDF form on the health status of The Jackson Laboratory animal rooms G3A and G3B (The Mouse Heart, Lung, Blood and Sleep Disorders Center research facilities) please click the document links below:
G3A - Click here for details on Room Health Status
G3B - Click here for details on Room Health Status
